Optimization of Retention Time of Microbial Community Structure of Activated Sludge Process

M P. Shah

American Journal of Water Resources. 2014, 2(6), 149-158
DOI: 10.12691/AJWR-2-6-3

Original Research

Optimization of Retention Time of Microbial Community Structure of Activated Sludge Process

M P. Shah^1,

¹Industrial Waste Water Research Laboratory Division of Applied & Environmental Microbiology Enviro Technology Limited Gujarat, India

Pub. Date: December 17, 2014

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Cite this paper

M P. Shah. Optimization of Retention Time of Microbial Community Structure of Activated Sludge Process. American Journal of Water Resources. 2014; 2(6):149-158. doi: 10.12691/AJWR-2-6-3

Abstract

Ammonia Oxidizing Bacteria community composition was analysed using fluorescence in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE), and the identified populations were enumerated by quantitative FISH. Potential nitrification rates were determined in batch tests and the in situ rates were calculated from mass balances of nitrogen in the plants. Increased SRT did not reduce the nitrification activity, but the number per mixed liquor suspended solids nor was community composition of AOB affected. Two dominant AOB populations related to Nitrosomonas europaea and Nitrosomonas oligotropha were identified by FISH, whereas only the latter could be detected by DGGE. The effect of a longer SRT on the activity was probably because of physiological changes in the AOB community rather than a change in community composition.

Keywords

activated sludge, ammonia-oxidizing bacteria, denaturing gradient gel electrophoresis, fluorescence in situ hybridization, nitrification, nitrogen removal, solids retention time

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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